diff --git a/lab-service/lab-lims/src/main/java/org/springblade/lims/service/impl/EntrustServiceImpl.java b/lab-service/lab-lims/src/main/java/org/springblade/lims/service/impl/EntrustServiceImpl.java
index 5db1d38..f4d4610 100644
--- a/lab-service/lab-lims/src/main/java/org/springblade/lims/service/impl/EntrustServiceImpl.java
+++ b/lab-service/lab-lims/src/main/java/org/springblade/lims/service/impl/EntrustServiceImpl.java
@@ -1324,9 +1324,11 @@ public class EntrustServiceImpl extends BaseServiceImpl<EntrustMapper, Entrust>
             PCR2Excel pcr2Excel1 = new PCR2Excel();
             PCR2Excel pcr2Excel2 = new PCR2Excel();
             pcr2Excel1.setExperieNum("阴性数量");
-            pcr2Excel1.setCtValue(String.valueOf(yin));
+            // 减1是因为，不能把阴性对照的阴性结果当做样品计算
+            pcr2Excel1.setCtValue(String.valueOf(yin - 1));
             pcr2Excel2.setExperieNum("阳性数量");
-            pcr2Excel2.setCtValue(String.valueOf(yang));
+            // 减1是因为，不能把阳性对照的阳性结果当做样品计算
+            pcr2Excel2.setCtValue(String.valueOf(yang - 1));
             list.add(pcr2Excel1);
             list.add(pcr2Excel2);